Workers at Olympic Dam in the outback will need to be fully vaccinated as a condition of entry from early next year.
Miner BHP has the made the decision after a thorough review of the effectiveness of its COVID-19 health and safety controls, the latest scientific evidence and expert health advice.
The rule will come into effect by January 31 2022 and will also be a condition of entry to people visiting the site too.
BHP Minerals Australia President Edgar Basto said: "the science is clear that widespread vaccination saves lives."
"In line with Government guidance, we recognise the path forward is through widespread vaccination in Australia and we are looking at a range of practical ways to support that while protecting communities and workforces.
"We have undertaken a thorough assessment and believe that this is the right path forward to protect the health of our people, their families and the communities where we operate including remote Indigenous communities while continuing to safely run our operations.
"We know this raise will questions for some, and we will work closely with our workforce as we go through the process of implementing these controls at our workplaces."
This follows as the mining giant has started to begin trialling workplace-based vaccinations in Roxby Downs.
BHP is now working with SA Health to establish a mobile clinic at Olympic Dam to help bolster vaccination rates for the workers before the rule comes into effect next year.
"We have to work together in the fight against the COVID-19 Delta strain in Australia, and we are determined to play our part," Mr Basto said.
"Getting more Australians vaccinated more quickly will help protect communities and enable a safe return to more normal ways of working across all sectors and regions, benefiting small and large businesses alike.
"We have an obligation to ensure our people are safe when they are at work, and to support the health of the regional communities where we operate."
This follows from BHP's successful onsite clinic used in Chile.